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HCC70CellMorphology

HCC70 (Human breast cancer cell line) Whole Cell Lysate, 100ug, Biomiga


List price: $120.00  

$108.00

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Biomiga
CL-002
In stock
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HCC70 (Human breast cancer cell line) Whole Cell Lysate, 100ug, Biomiga

Biomiga provides researchers with a variety of ready-to-use cell line lysates for proteomics research. Biomiga lysates are available for applications including electrophoresis, western blot, immunoprecipitation, enzymatic activity analysis, protein-protein interaction analysis, and tissue specific expression identification. Please check product documentation to see recommended and tested applications for each cell line lysate.

Tested applications 

WB

Suggested applications 

WB, ELISA, Immunoprecipitation

GAPDH western blot using HCC70 lysate.

                    gapdh_western_blot_using_hcc70_lysate_347

Recommended dilutions

HCC70 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

Relevance 

HCC70 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.

Purity 

Whole Cell Lysate

Form 

It is supplied as whole cell lysate, in RIPA buffer with protease inhibitors and phosphotase inhibitors.

Concentration 

2.000 mg/ml

Size:

100ug

Storage instructions 

Shipped on dry ice. Upon delivery aliquot and store at  -80°C. Avoid repeated freeze / thaw cycles.

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