MDA-MB-468 Cell Morphology, Biomiga

MDA-MB-468 (Human breast cancer cell line) Whole Cell Lysate, 100ug, Biomiga


 

$70.00

Biomiga
CL-001
In stock
+

MDA-MB-468 (Human breast cancer cell line) Whole Cell Lysate, Biomiga

Biomiga provides researchers with a variety of ready-to-use cell line lysates for proteomics research. Biomiga lysates are available for applications including electrophoresis, western blot, immunoprecipitation, enzymatic activity analysis, protein-protein interaction analysis, and tissue specific expression identification. Please check product documentation to see recommended and tested applications for each cell line lysate.

Description 

MDA-MB-468 (Human breast cancer cell line) Whole Cell Lysate

Tested applications 

WB

Suggested applications 

WB, ELISA, Immunoprecipitation

Size:

100ug

Recommended dilutions

MDA-MB-468 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

Relevance 

MDA-MB-468 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.

Purity 

Whole Cell Lysate

Form 

It is supplied as whole cell lysate, in RIPA buffer with protease inhibitors and phosphotase inhibitors.

Concentration 

2.000 mg/ml

Storage instructions 

Shipped on dry ice. Upon delivery aliquot and store at  -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Cell line: MDA-MB-468 (Human breast adenocarcinoma)
Growth media: RPMI1640 & 10% FCS (Fetal Calf serum)

GAPDH western blot using MDA-MB-468 lysate.

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