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KUIQPICK v.1.0 is a capillary-based vacuum-assisted device for tissue microdissection and collection of cells from cell culture dishes. High quality RNA, protein, and DNA are isolated from dissected samples, suitable for genomics and proteomics applications.
The advantage of KUIQPICK is its cost effectiveness, flexibility and ease of use compared to existing technologies, such as LCM and FACS.
Using the X-Y mechanical stage the tissue/cells of interest are located. A single press of a button initiates collection of the tissue area/cells into the disposable capillary unit (DCU). Collected samples are transferred to a test tube for further experimentation.
KUIQPICK is perfectly suitable for the collection of live cells and cell clusters. It collects cells from adherent cultures and subanatomical areas from live brain tissues without affecting their viability so that the collected cells can be used for further culturing.
KUIQPICK can be applied for the collection of various types of cells grown as adherent cell cultures. Individual cells may be collected for their further experimentation, such as microarray based gene expression analysis, or for recultivation. Please see Protocols below.
Original concept of Kuiqpick and some of its applications in several research areas are described in the original article published in 2012. For more detailed protocols please refer to the Application Notes below. In addition, a new application of Kuiqpick for the collection of individual cells from adherent cell cultures is described (Protocol 3) below.
Isolation of specific subanatomical regions or single cells in the central nervous system is a delicate procedure requiring careful preparation of tissue specimen. In areas with low cell density singe cells may be collected using Kuiqpick, while in areas with high density of cells within a cellular layer (e.g. granular cell layers of the cerebellum, dentate gyrus, or CA1-3 areas of the hippocampus) clusters consisting of the same cell types may be collected with each single acquisition step†. Separation of cell bodies and dendrites can also be performed in certain areas of the brain, such as the hippocampus and cerebellum. This protocol describes the isolation of single cells, cell clusters, and subanatomical regions from sucrose treated brain slices. Sample microdissections are provided to demonstrate the effectiveness of this method.
Kuiqpick v.1.0 is an efficient and effective way for the isolation of subanatomical regions from live brain tissue slices. The vacuum pulls the cells into the capillary with minimal mechanical damage, and large areas can be collected quickly using disposable capillaries (DCU) with greater inner diameters. This protocol describes the technique established to isolate living cells from live brain sections for primary neural progenitor cell culture. A simple protocol for the collection of various subanatomical brain regions and the successful subsequent recultivation of stem cell cultures is described here.
This application note is aimed at demonstrating the performance of Kuiqpick on adherent cell cultures and determined that this is a suitable method for individual cell collection and subsequent clonal expansion. Kuiqpick also accurately and rapidly acquired both fluorescently labeled and non-labeled individual cells directly from standard culture dishes, and more importantly, the collected cells were suitable for subsequent recultivation.
Below are the sample videos of cell and tissue collection process using Kuiqpick v.1.0. The videos show both collection of the specific tissue areas and individual cells from brain tissues and adherent cell cultures.
Video 1: Sample microdissection of mouse hippocampal subanatomical regions from 50 μm sucrose treated fresh frozen tissue sections using Kuiqpick v.1.0.
Video 2: Sample collection of individual neurons from 20 μm sucrose treated fresh frozen mouse brain sections and SH-Sy5Y human neuroblastoma cell lines using Kuiqpick v.1.0.
Protocol 1 Microdissection of Brain Tissues Using KuiqpicK (Protocol_1._Microdissection_of_brain_tissues.pdf, 218 Kb) [Download]
Protocol 2. Collection of Neural Progenitors from Primary Brain Tissues Using KuiqpicK (Protocol_2._KuiqpicK_for_collection_of_neural_progenitors.pdf, 169 Kb) [Download]
Protocol 3. Collection of Individual Cells and their Clonal Expansion from Adherent Cell Cultures (Protocol_3._Cell_collection_and_expansion.pdf, 354 Kb) [Download]
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