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Biomiga

Virus RNA Kit, 50 Preps, Biomiga


List price: $160.00  

$144.00

You save: $16.00 (10%)
Biomiga
R6620-01
In stock
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The EZgeneTM Viral RNA Kit provides an easy and reliable method for isolating total viral RNA from plasma or serum while enzyme inhibitors and other contaminates completely removed. This procedure has been tested for isolating nucleic acids from Hepatitis A, Hepatitis B, Hepatitis C and HIV. This kit can also be used to isolate viral DNA/RNA from urine and cell culture supernatant.

Storage and Stability

All other components can be stored at room temperature. All kit components are guaranteed for 12 months from the date of purchasing.

Kit Contents

Catalog#

R6620-00

R6620-01

R6620-02

Preps

4

50

250

Buffer LY

2.4 mL

28 mL

135 mL

Buffer RB

3 mL

40 mL

185 mL

RNA Wash Buffer

2 mL

20 mL

3 x 24 mL

DEPC-Treated dH2O

500 æL

10 mL

30 mL

ezBind Columns

4

50

250

Collection Tubes

8

100

500

User Manual

1

1

1

Before Starting

Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.

Important

  • Determine the volume of Buffer LY to be used and add 20 æL of ?-mercaptoethanol (?-ME) per 1 mL Buffer LY before use. Buffer LY contains ?-ME can be stored at room temperature for up to 1 month.
  • Crystals may form in Buffer LY, dissolve the precipitates at 37 oC before use.
  • Add 8 mL (R6620-00), 80 mL (R6620-01) or 3 x 96 mL (R6620-02) 100% ethanol to RNA Wash Buffer before use. The ethanol is 80%.

Materials supplied by users

  • Tabletop microcentrifuge and 1.5 mL sterile tubes.
  • Vacuum manifold if use vacuum protocol.
  • 100% ethanol.

Note: Perform all steps including centrifugation at room temperature

Trouble Shooting Guide

Problem

Possible reason

Suggested Improvement

Low A260/A280ratios

Protein contamination

Do a Phenol: Chloroform extraction. Loss of total RNA (up to 40%) should be expected.

Guanidine Thiocyanate contamination

Add 2.5 volumes of ethanol and 0.1M NaCl (final concentration) to precipitate RNA. Incubate for 30 min at -20 oC.. Centrifuge at 10,000 g for 15 min at 4 oC.. Resuspend the RNA pellet in DEPC-treated water.

Low Yield

RNA in sample degraded

Freeze samples immediately in liquid nitrogen and store at -70 oC. after collect it.

The binding capacity of the membrane in the spin column was exceeded

Use of too much tissue sample exceeding the binding capacity of spin column will cause the decreasing of total RNA yield.

Ethanol not added to buffer

Add ethanol to the RNA Wash Buffer before purification.

Operating Protocol ?

r662033521_448

None