Biomiga's One-Step RT-PCR is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA. This system uses a mixture of MMLV Reverse Transcriptase and Hot start Taq DNA polymerase in an optimized reaction buffer, and can detect RNA targets up to 4.0 kb. The amount of input total RNA can range from 0.01 pg to 2 μg. Sufficient reagents are provided for 25 or 100 amplification reactions of 50 μl each.
Convenient one-tube setup
Optimized RT-PCR buffer and robustness
High sensitivity, specificity, and reproducibility
MMLV Reverse Transcriptase
Hot start Taq DNA polymerase
|RNA Target Size|
up to 4.0 kb
|Sample Type (General):|
RNA (total RNA or mRNA)
|Input Total RNA|
0.01 pg to 2 μg
For Research Use Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Enzyme mix: MMLV RT/ Hot start Taq Mix
2X Reaction mix (optimized buffer contains dNTP and Mg)
5 mM Magnesium Sulfate
Store at -20°C，One-Step RT-PCR is stable for 2 years when stored at -20°C.
Biomiga One Step RT PCR Kit RT0213 User Guide by GeniCity (userguideRT0213-02_G1_august7_13002.pdf, 521 Kb) [Download]