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One-Step RT-PCR Kit, 25 Preps, Biomiga

List price: $150.00  


You save: $15.00 (10%)
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Biomiga's One-Step RT-PCR is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA. This system uses a mixture of MMLV Reverse Transcriptase and Hot start Taq DNA polymerase in an optimized reaction buffer, and can detect RNA targets up to 4.0 kb. The amount of input total RNA can range from 0.01 pg to 2 μg. Sufficient reagents are provided for 25 or 100 amplification reactions of 50 μl each.


  • Convenient one-tube setup
  • Optimized RT-PCR buffer and robustness
  • High sensitivity, specificity, and reproducibility






PCR Method:

1-Step qRT-PCR 

Reverse Transcriptase:

MMLV Reverse Transcriptase 


Hot start Taq DNA polymerase

Detection Method:

Primer-Probe Detection 

RNA Target Size

up to 4.0 kb

Sample Type (General):

RNA (total RNA or mRNA)

Input Total RNA

0.01 pg to 2 μg


25 Preps 

cGMP Compliance:

non-cGMP Compliant 

Shipping Condition:

Blue Ice

Regulatory Statement:
For Research Use Use Only. Not intended for any animal or human therapeutic or diagnostic use.


Kit Contents



Enzyme mix: MMLV RT/ Hot start Taq Mix

25 μl

2X Reaction mix (optimized buffer contains dNTP and Mg)

0.65 ml

5 mM Magnesium Sulfate

500 μl









Store at -20°C,One-Step RT-PCR is stable for 2 years when stored at -20°C.

Biomiga One Step RT PCR Kit RT0213 User Guide by GeniCity (userguideRT0213-02_G1_august7_13002.pdf, 521 Kb) [Download]

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