Adenovirus Purification Maxi Kit, Biomiga

The ViraTrapTM Adenovirus Purification Maxi Kit is designed for fast and efficient purification of recombinant adenovirus from adenovirus transfected cell culture supernatant. Up to 4 x1012 viral particles can be purified from cell culture media of 6 to 8 x T75 flasks.

Traditionally the recombinant adenovirus is purified by ultra centrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed.

Each column can be regenerated for purifying the same adenovirus. For optimized viral binding and recovery, each column can be regenerated only once.

 

 Featured Citations

Alantolactone Inhibits Cell Proliferation by Interrupting the Interaction between Cripto-1 and Activin Receptor Type II A in Activin Signaling Pathway

J Biomol Screen, 2011, March 4, 2011 1087057111398486

 

Deletion of CaMKK2 from the Liver Lowers Blood Glucose and Improves Whole-Body Glucose Tolerance in the Mouse.

Molecular Endocrinology January 12, 2012 me.2011-1299

 

 

Kit Contents

Catalog#

V1260-00

V1260-01

V1260-02

Notes

Preps

2

4

10

 

Maxi Columns

1

2

5

Can be used twice

(Store at 4°C)

Press-On Cap

1

2

5

Store at RT

Desalting Tube*

Not Included

1

1

Can be regenarated

(Store at 4°C)

50 mL Collection Tube

2

4

10

Store at RT

10 x Wash Buffer

6 mL

30 mL

60 mL

Store at RT

2 x Elution Buffer

6 mL

30 mL

60 mL

Store at RT

Regeneration Buffer

6 mL

30 mL

60 mL

Store at RT

*Desalting tube could be regenarated and applied on one kind of virus. Desalting tube could be purchased from Biomiga.

 

Safety Consideration

The adenovirus infected cell media and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition.

 

Storage and Stability

All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).

 

Materials required but not supplied 

  • ddH2O
  • PBS
  • 0.45 μm and 0.22 μm filters
  • Rack holder for columns