On-chip plasma generation out of whole blood

The membrane chip enables you to generate blood plasma from 20-40 µl of whole blood (stabilized or non-stabilized) within less than 2 minutes. The yield is roughly 50% of plasma. A special membrane inside the chip retains all blood cells. The pure plasma migrates through the filter.

Required tools & ingredients
1. Chip with 4 plasma generation membranes (15-1504-0200-02)
2. Mini Luer plugs (09-0550-0000-09)
3. Eppendorf vessel

The application procedure includes three steps:
1. Preparation of the chip
2. Sample loading
3. Filtration

Preparation steps:
The ventilation ports of the membrane chip are closed with Mini Luer plugs and the chip is placed on a bench in the shown orientation.

Sample loading:
Pipette the designated volume (between 20 and 40 µl) of whole blood into Luer-inlet-port of the membrane chip.

Filtration:
Use a pipette (for yellow tips) with a set volume of 100 µl. Press the pipette tip tightly into the sample outlet port and suck slowly for ~30 sec. Formation of air bubbles during filtration is normal and has no effect on the generated plasma. Fill the filtrated plasma into a fresh Eppendorf tube.

Results:
Based on your starting volume, between 10 and 20 µl of blood plasma will be generated. It should be clear, light yellow and free of blood cells.

There are no products in this section