How to work with olive interfaces

Olive interfaces are simple connectors to be manually connected with tubes like the best known example of our daily life, the hose pipes. Tubes can be directly connected to such chips. They are well suited for manual handling, but automated approaches moving the silicone sleeve over the olive are possible as well, even if difficult to realize. This chapter summarizes the different options to work with olive interfaces on chip and how to operate chips with such interfaces.

Hints to work with olive interfaces on chip:
Olive interfaces connected through silicones sleeves and PTFE tube to pump
Required item:
1. Microfluidic chip with Luer interface, e.g. micro mixer chip (01-0190-0138-01)
2. Handling frame, e.g. orange (15-4001-0000-12)
3. Silicone tube, e.g. ID: 0.5 mm (09-0802-0000-00)
4. PTFE tube, e.g. ID: 0.5 mm (09-0803-0000-00)
5. Peristaltic pump
6. Tube for peristaltic pump
7. Eppendorf vessel

Step 1: Chip & handling frame
1. Insert the chip in a handling frame

Step 2: Connect PTFE tubes with silicone sleeves
2. Connect two times a short silicone tube with a longer PTFE tube

Step 3: Interface chip & tube
3. Interface the olives on chip through the silicone sleeves with the PTFE tube

Step 4: Insert tube in pump tube
4. Insert the PTFE tube in the tube of the pump

Step 5: Tube, pump & reservoir vessel
5. Connect the tube of the pump with a PTFE tube with the reservoir vessel

Step 6: Connection collection vessel & start pumping
6. Connect the exit tube with a collection vessel and start pumping

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