How to work with Luer interfaces

This chapter summarizes the different options to work with Luer interfaces on chip and how to operate chips with such interfaces.

Hints to work with female Luer interfaces on chip:
Option 1: Female Luer interface as pipetting interface or reservoir
The most simple option how to use chips with female Luer interface is to insert the liquid with a standard syring.
Required item:
1. Microfluidic chip with Luer interface
2. Standard syringe

Option 2: Female Luer interface as pipetting interface or reservoir
Another option how to use chips with female Luer interface is to insert the liquid with a pipette or to use the female Mini Luer interfaces as reservoirs.
Required item:
1. Microfluidic chip with Luer interface
2. Conventional pipette

Option 3: Female Luer interface combined with male Luer counterpart
Required item:
1. Microfluidic chip with Luer interface, e.g. micro mixer chip (14-1035-00186-01)
2. Handling frame, e.g. orange (15-4001-0000-12)
3. Male Luer fluid connectors, e.g. the green version (09-0509-0000-09)
4. Male Luer plugs, e.g. the black version (09-0504-0000-09)
5. Silicone tube, e.g. ID: 0.5 mm (09-0802-0000-00)
6. PTFE tube, e.g. ID: 0.5 mm (09-0803-0000-00)
7. Peristaltic pump
8. Tube for peristaltic pump
9. Eppendorf vessel

Step 1: Chip & handling frame
1. Insert the microfluidic chip in a handling frame for microfluidic chips in microscopy slide format

Step 2: Luer connector & silicone sleeve
2. Interface the Luer fluid connector with a small piece of silicon tube

Step 3: Silicone sleeve & PTFE tube
3. Interface the Luer fluid connector with the mounted silicone sleeve with the PTFE tube

Step 4: Insert connector on chip
4. Insert the Luer fluid connector connected with silicone sleeve and PTFE tubing with a twist on the female interface on chip

Step 5: Insert connector on exit & connect to collection vessel
5. Insert a second Luer fluid connector connected with silicone sleeve and PTFE tubing with a twist on the female interface on chip and place the end of the PTFE tube in an Eppendorf vessel for sample or waste collection

Step 6: Close unused ports with plugs
6. Close all unused fluid entrance and fluid exit ports of the fluidic pathway used on chip with Luer plugs.

Step 7: Connect chip with pump
7. Connect the PTFE tube with the tube inserted in the pump

Step 8: Connect pump with reservoir and start pumping
Female Luer interface combined with male Luer counterpart
8. Connect the end of the pump tube with a further PTFE tube, insert the PTFE tube in your reagent vessel and start pumping.

There are no products in this section