How to work with Mini Luer interfaces

This chapter introduces how to work with Mini Luer interfaces and how to operate chips with such
interfaces.

Hints to work with female Mini Luer interfaces on chip:
Option 1: Female Mini Luer interface as pipetting interface or reservoir
The most simple option how to use chips with female Mini Luer interface is to insert the liquid with a pipette or to use the female Mini Luer interfaces as reservoirs.

Required item:
1. Microfluidic chip with Mini Luer interface
2. Conventional pipette

Option 2: Female Mini Luer interface combined with male Mini Luer counterpart

Required item:
1. Microfluidic chip with Mini Luer interface, e.g. micro mixer chip (14-1039-0286-01)
2. Handling frame, e.g. orange (15-4001-0000-12)
3. Male Mini Luer fluid connectors, e.g. the green version (09-0541-0000-09)
4. Male Mini Luer plugs, e.g. the red version (09-0551-0000-09)
5. Silicone tube, e.g. ID: 0.5 mm (09-0802-0000-00)
6. PTFE tube, e.g. ID: 0.5 mm (09-0803-0000-00)
7. Peristaltic pump
8. Tube for peristaltic pump
9. Eppendorf vessel

Step 1: Chip & handling frame
1. Insert the microfluidic chip in a handling frame for microfluidic chips in microscopy slide format

Step 2: Mini Luer connector & silicone sleeve
2. Interface the Mini Luer fluid connector with a small piece of silicon tube

Step 3: Silicone sleeve & PTFE tube
3. Interface the Mini Luer fluid connector with the mounted silicone sleeve with the PTFE tube

Step 4: Insert connector on chip
4. Insert the Mini Luer fluid connector connected with silicone sleeve and PTFE tubing with a twist on the female interface on chip

Step 5: Insert connector on exit & connect to collection vessel
5. Insert a second Mini Luer fluid connector connected with silicone sleeve and PTFE tubing with a twist on the female interface on chip and place the end of the PTFE tube in an Eppendorf vessel for sample or waste collection

Step 6: Close unused ports with plugs
6. Close all unused fluid entrance and fluid exit ports of the fluidic pathway used on chip with Mini Luer plugs.

Step 7: Connect chip with pump
7. Connect the PTFE tube with the tube inserted in the pump

Step 8: Connect pump with reservoir and start pumping
8. Connect the end of the pump tube with a further PTFE tube, insert the PTFE tube in your reagent vessel and start pumping.

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