Lentivirus Purification Maxi Kit, Biomiga

The ViraTrapTM lentivirus maxi purification kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. 3x1011 Viral particles can be purified from cell culture of 4 to 5 T75 flasks per column.

The viruses are first applied to a purification column and then further purified and concentrated through a concentration unit.

Each column can be regenerated for purifying the same lentivirus. For optimized viral binding and recovery, each column can be regenerated only once.

 

Features

  • ViraTrap Maxi Lentivirus Purification Kit can concentrate up to 50 -100 fold.

  • The maximum load of ViraTrap Maxi Lentivirus Purification Kit is 15x6 mL.

  • High purity: the samples are purified by two different columns.

  • Lentivirus Purification Kit columns can be regenerated for a second use after a buffer wash.

 

Kit Contents

Catalog#

V1270-01

V1270-02

Preps

4

10

LV Columns

2

5

Press-On Cap

4

10

Centrifugal Device

4

10

50 mL Conical Tube

8

20

Buffer P

80 mL

180 mL

Buffer S

80 mL

180 mL

Regeneration buffer

20 mL

40 mL

 

Storage and Stability

The Guaranteed shelf life is 12 months from the date of purchase. Store LV column at 4°C

 

Safety Considerations 

The lentiviral infected cell culture and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition.

 

Materials Required but Not Supplied

  • Standard TC centrifuge
  • Swing bucket rotor.
  • 0.45 μm filter unit
  • Rack holder for column   

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