Lentivirus Purification Mini Kit, Biomiga

 

The ViraTrapTM lentivirus mini purification kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Up to 1x1011 viral particles can be purified from cell culture media of 1 to 2 T75 flasks.

The viruses are first pelleted from viral supernatant and then further purified and concentrated through a purification column and a desalting/concentration unit. 

Each column can be regenerated for purifying the same lentivirus. For optimized viral binding and recovery, each column can be regenerated only once.

 

 

Features

  • ViraTrap Mini Lentivirus Purification Kit can concentrate up to 50 -100 fold.

  • High purity: the samples are purified by two different columns.

  • Lentivirus Purification Kit columns can be regenerated for a second use after a buffer wash.

 

Kit Contents 

Catalog# V1170-01 V1170-02
Preps 10 20
LV Columns 5 10
Press-On Cap 10 20
Centrifugal Device 5 10
15 mL Conical Tube 10 20
Buffer P 50 mL 100 mL
Buffer S 50 mL 100 mL
Regeneration Buffer 25 mL 50 mL

*  Centrifugal device can be purchased from Biomiga separated.

 

Storage and Stability

The Guaranteed shelf life is 12 months from the date of purchase. Store LV column at 4°C

 

Safety Considerations

The lentiviral infected cell culture and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition.

 

Materials required but not supplied 

  • Standard TC centrifuge
  • Swing bucket rotor.
  • 0.45 μm filter unit
  • Rack holder for column
  • PBS