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Retrovirus Purification Maxi Kit, Biomiga
Biomiga's ViraTrapTM Retrovirus Maxi Purification Kit is designed for fast and efficient purification of recombinant retroviruses from retrovirus-transfected cell culture supernatant. Up to 1 x1013 viral particles can be purified from cell culture media of 6 to 8 T75 flasks.
Traditionally the recombinant retrovirus is purified by ultracentrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed.
Each column can be regenerated for purifying the same retrovirus. For optimized viral binding and recovery, each column can be regenerated only once.
Citations
Mol Vis (2010). 2010; 16: 310–319.
I. J. Immunol. September 2010, 7 (3), 162-176
Kit Contents
|
Catalog# |
Notes |
||
|
Preps |
10 |
20 |
|
|
Press-On Cap |
5 |
10 |
Store at RT |
|
Maxi Columns |
5 |
10 |
Can be used twice(Store at 4°C) |
|
Desalting Tube* |
1 |
1 |
Can be regenarated(Store at 4°C) |
|
15mL Collection Tube |
10 |
20 |
Store at RT |
|
10 x Wash Buffer |
40 mL |
80 mL |
Store at RT |
|
2 x Elution Buffer |
40 mL |
80 mL |
Store at RT |
|
Regeneration Buffer |
40 mL |
80 mL |
Store at RT |
*Desalting tube could be regenarated and applied on one kind of virus. Desalting tube could be purchased from Biomiga.
Storage and Stability
All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).
Safety Considerations
The retrovirus infected cell media and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition.
Materials Required But Not Supplied
ddH2O, PBS, 0.45 μm filters, Rack holder for columns
