Assay chip 2 – turning valve assisted fluid control with separate assay and reference cavities

This integrated chip allows for the development of hybridization assays on chip. Six 12 μl cavities
with 200 μm depth can be used for spotting different kinds of arrays. Four of these chambers are are
operated in row, two further separately. The separate chambers allow e.g. for control or quantification
reactions and have pre-cavities to store dry reagents.
Sample injection is foreseen through a female Luer interface, reagent supply or air pressure through
the female Mini Luer interfaces.
The chip is equipped with the following main elements:
• 6 reaction & detection chambers with 12 μl volume
• 2 pre-storage chambers with 12 μl volume
• 2 turning valves
The liquids are controlled with the help of two turning valves, sample injection is foreseen through a
female Luer interface, reagent supply or air pressure through the female Mini Luer interfaces.
To operate this chip Luer and Mini Luer male fluid connectors, Luer and Mini Luer plugs as well as
silicone and Teflon tubings are of use to allow for the connection of the chip with pumps. Direct
filling of the chip with a pipette is possible, the use of pipette connectors is appreciated by several
operators. The turning valves can be rotated with a special manual turning valve manipulator. All these
accessories are combined in the integrated chip support kit 1.