A 300 µl deep central chamber with 30 µl inner volume is the reaction cavity of this chip. To keep the components in the chamber either magnetic beads can be used which need to be operated by an external magnet or catcher molecules have to be immobilized on the surface of the cavity. Controlled by two turning valves liquids can be supplied and removed, air pressure can be applied and venting can be ensured.
Liquid supply and air pressure are foreseen through the female Mini Luer interfaces on chip. The chip is equipped with the following main elements:
• Cavity with 30 µl volume
• 2 turning valves
To operate this chip Mini Luer male fluid connectors, Mini Luer plugs as well as silicone and Teflon tubings are of use to allow for the connection of the chip with pumps. Direct filling of the chip with a pipette is possible, the use of pipette connectors is appreciated by several operators. The turning valves can be rotated with a special manual turning valve manipulator. All these accessories are combined in the integrated chip support kit 3.