This integrated chip allows for the development of biological assays on chip. The chip enables for on- chip metering, mixing and the detection of the reaction in a separate chamber.
For this purpose the chip is equipped with the following main elements:
• Metering cavity with 15 µl volume
• Metering loop with 17 µl volume
• Mixing chamber with 102 µl volume
• Reaction & detection chamber with 20 µl volume
• Two turning valves
The liquids are controlled with the help of two turning valves, overfilling of the detection chamber is prevented through a liquid tight membrane, the sample can be introduced through the female Luer interface and further reagents or air pressure can be supplied through the female Mini Luer interfaces. To operate this chip Luer and Mini Luer male fluid connectors, Luer and Mini Luer plugs as well as silicone and Teflon tubings are of use to allow for the connection of the chip with pumps. Direct filling of the chip with a pipette is possible, the use of pipette connectors is appreciated by several operators. The turning valves can be rotated with a special manual turning valve manipulator. All these accessories are combined in the integrated chip support kit 1.