Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity at ambient temperature. Once the PCR step reaches denaturation temperature (94℃), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield. The use of this antibodybased hot start enzyme complex allows for convenient room temperature reaction set-up and reduces PCR optimization effort and contamination risk.
Hot Start Taq DNA polymerase has intrinsic 5’->3’ DNA polymerase activity and 5’->3’ exonuclease activity but lacks 3’->5’ exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa and displays optimal activity at a temperature between 70-74℃. The enzyme is provided with 10XPCR buffer and 50 mM MgCl2 solution to perform PCR amplification.
Storage and Stability
Hot Start Taq DNA polymerase is stable for 2 years when stored at -20°C.
• Room temperature reaction set-up
• Automatic hot start PCR
• High sensitivity, high specificity, and high yield
• Superior reliability and robustness
• Ideal for everyday PCR