Western Blotting Substrates, Biomiga

ECL Western Blotting Substrate

·       Higher sensitivity

·       Superior signal

·       Clear background

·       Easy to use

 

Product contents

Catalog#

EC1808-25

EC1808-100

EC1808-500

Reagent A

25 mL

100 mL

5x100 mL

Reagent B

125 µL

500 µL

5x500 µL

Sufficient membrane

250cm2

1000cm2

5000cm2

 

Western Blotting Analysis (5 seconds exposure)

Biomiga Western Blotting Substrate

Western blot analysis of diluted HeLa cell lysates using anti-β-Actin antibody. Lane 1 contained 2μg of total protein, and four 2-fold serial dilutions were applied to lanes 2-5 at 10μL/well.

 

Procedure Summary

1.       Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBS/T.

2.       Prepare 1X ECL Western Blotting Substrate by mix Reagent A and Reagent B at a 1000:5 ratio (i.e., 5mL Reagent A + 25μL Reagent B for 6 × 8 cm2membrane) and add it to the blot.

3.       Incubate Reagent with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic.

4.       Expose blot to X-ray film.