Two Step qPCR Kit, SYBR Green QuickMix®, Biomiga

Biomiga® SYBR® Green QuickMix, a 2X concentrated, ready-to-use reaction mix that contains all components, except primers and template for real-time quantitative PCR on Applied Biosystems 7000, 7300, 7700, or 7900 instruments and Bioers Line-Gene K Instruments. This unique combination of buffer, stabilizers, and Taq DNA polymerase deliver maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR® Green qPCR. Highly specific amplification is critical to successful qPCR with SYBR® Green I dye technology because this dye binds to and detects any double stranded DNA generated during amplification. Taq DNA polymerase is a key component of this reaction mix. This hot-start Taq contains a mixture of monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step (> 48 hours at room temperature). Similar to our Taq DNA polymerase, these antibodies are irreversibly inactivated during the initial PCR denaturation step. However, unlike other antibody hot-start polymerases, activation of Taq is instantaneous at 95°C. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Replication of fragments up to 200 bp is complete in less than 20s at 60°C.

 

Real-Time PCR Instrument Compatibility

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Bioer Line-Gene K

 

Contents

  • 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuFast Taq DNA Polymerase, SYBR Green I dye, ROX Reference Dye, and stabilizers.

 

Storage conditions

Biomiga SYBR Green QuickMix, is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

 

Precautions and Disclaimer

For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.