One-Step qRT-PCR Kit, SYBR Green ExpressMix Biomiga

The Biomiga One-Step  qRT-PCR SYBR Green ExpressMix Kit is a fast, convenient and highly sensitive solution for quantitative RT-PCR of RNA templates (qRT-PCR) using SYBR Green I dye detection and gene-specific primers on all Applied Biosystems real-time PCR systems (except the 7500) and Bioer's LineGeneK. cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. The system has been optimized to deliver optimal RT PCR efficiency, sensitivity, and specificity. The reaction buffer has been specifically formulated to optimize activities of both reverse transcriptase and Taq DNA polymerase while minimizing primer-dimer and other non-specific PCR artifacts. The kit is designed for both fast and standard qPCR cycling protocols. Highly specific amplification is essential for successful qRT-PCR with SYBR Green I technology, since this dye binds to any dsDNA generated during amplification. Biomiga Taq DNA polymerase contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation at 95oC, the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase.

 

Real-Time PCR Instrument Compatibility

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Bioer LineGeneK

Contents

  • qScript One-Step Reverse Transcriptase - Optimized 50X formulation of recombinant MMLV reverse transcriptase for one-step RT-PCR.
  • One-Step SYBR Green Master Mix, - 2X reaction buffer containing dNTPs, magnesium chloride, Taq DNA polymerase, stabilizers, ROX reference dye and SYBR Green I dye
  • Nuclease-free water

Storage conditions

Kit components are stable for one year when stored in a constant temperature freezer at 20oC, protected from light. For convenience, the One-Step SYBR Green Master Mix  may be stored unfrozen at +2 to +8oC for up to 4 months.

Precautions and Disclaimer

For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.