EndoFree Plasmid ezFilter Maxiprep, Biomiga

The key elment to Biomiga's proprietary DNA binding system that allows the high efficient binding of DNA to our ezBindTM matrix while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer.

Unlike other kits in the markets, our patented plasmid purification kit has no chaotropic salts in the buffer. the purified DNA is guanidine/anion exchange resin residues free.

The EZgeneTM endofree system uses a specially formulated buffer that extracts the endotoxin from the bacterial lysate. Two rounds of extraction will reduce the endotoxin level to 0.1 EU (Endotoxin) per µg of plasmid DNA.

This kit is designed for fast and efficient purification of plasmid DNA from 150 to 200 mL of E. coli culture. The maxi column has a DNA binding capacity of 1200 µg. The purified endofree DNA is ready for downstream applications such as transfection of endotoxin-sensitive cell lines, primary cultured cells or microinjection.

Two endotoxin removal procedures are provided. Protocol A removes endotoxin during the purification of plasmid DNA and Protocol B removes endotoxin after the purification of plasmid DNA.

 

Kit Contents

Catalog #

PD1515-00

PD1515-01

PD1515-02

Preps

2

10

25

ezBindTM Columns

2

10

25

Filter syringe(60 mL)

2

10

25

Buffer A1

22 mL

110 mL

270 mL

Buffer B1

22 mL

110 mL

270 mL

Buffer N3

32 mL

160 mL

400 mL

RNase A(20 mg/mL)

2.2 mg(110 µL)

11 mg(550 µL)

27 mg(1.35 µL)

EndoClean Buffer

5 mL

25 mL

60 mL

Endofree Elution Buffer

5 mL

25 mL

60 mL

User Manual

1

1

1

 

Storage and Stability

Buffer A1 should be stored at 4°C once RNase A is added. All other materials can be stored at room temperature (22-25°C). The Guaranteed shelf life is 12 months from the date of purchase.