Cyanase Inactivation System
Superior Flexibility to Benzonase Nuclease
- Easy to Use
- No Optimization Needed
- Active Over a Wide Range of Conditions
- No Interation With Other Proteins or Targets
- Extremely Tight Interaction Between Cyanase and Resin
The image demonstrates the superior interaction of the resin with Cyanase. Various amounts of Cyanase were incubated with inactivation resin at room temperature and spun down to remove the resin. The resulting supernatants were incubated with 1 ug Lambda DNA for 1 hour at room temperature.
Cyanase Inactivation resin is a proprietary blend of Cyanase inhibitor protein coupled to Sepharose Fast Flow resin creating a novel method for inactivating and removing the Cyanase enzyme prior to downstream applications. No more worries about downstream contamination with nucleases or difficult removal processes which may compromise product integrity. Cyanase Inactivation resin makes the removal simple and easy to follow in as little as 20 minutes. To remove Cyanase, simply add 10l of 50% slurry for every 10 U of Cyanase or for every 100 l of sample, whichever is greater. The sample can then be inverted, pipetted or stirred for 20 minutes at room temperature to provide continuous mixing. The sample is then spun down at 4-5,000Xg. for 5 minutes and the soluble sample removed. Alternatively, less resin can be used if incubated for longer periods of time. For samples requiring more than 1 ml of resin slurry, the resin can be packed into a column and the sample passed over the resin.
Average Particle Size: 90 m in 50% buffer slurry.
Product Information: Inactivation resin is fully active under all normal Cyanase reaction conditions. In addition inactivation resin has been tested down to pH 4.8, up to pH 10.0, down to 0⁰C, and in 500 mM NaCl with no effect on Cyanase removal. Inactivation resin has no affinity for any other proteins or reagents.