Cyanase™ Nuclease System, RiboSolutions

Benzonase® Nuclease Alternative Cyanase™

Effective For

• Cell Lysate Clearance

• Protein and Viral Purification

• Nucleic Acid Removal from Samples

• Degrades all forms of DNA and RNA

Unique Features:

• Easily Inactivated and Removed

• Fastest Nuclease on the Market. BAR NONE

• Unsurpassed Stability. LAB TESTED UP TO 1 YEAR at room temperature with minimal loss of activity as measured by cell lysate clearance. No other competitor comes close.

The figure above demonstrates the superior activity of Cyanase™ versus leading market nucleases. 3 ug of Lambda DNA was incubated with various amounts of Cyanase™ and competitor enzymes for 1 minute at 37°C in their manufacturer recommended optimal buffers. Each reaction was then run on a 1% agarose gel. Only the Cyanase lanes demonstrate complete degradation of the target band at either unit amount.

Cyanase™ is a cloned highly active non-Serratia based non-specific endonuclease that degrades single and double stranded DNA and RNA in as little as 1 minute.

The Cyanase™ system is unique from Benzonase® nuclease in its ability to be easily removed from samples after the reaction is finished using the Cyanase™ inactivation resin. The resin can be easily filtered or spun down to remove from the sample removing the Cyanase™ with it.

Cyanase™ is active over several conditions and is fully active in DTT up to 100 mM, and most common non-ionic detergents up to 1% including Triton X-100, Tween 20, and Igepal CA630. Cyanase™ is active in Urea up to 3 M. Cyanase™ is unaffected by chemical lysate treatments such as lysozyme or detergent. Click the links below for ordering information.

Concentration: 50 U/µl

Purity: No contaminating bands detected by loading 5 ug of Cyanase on a 4-20% Tris-Glycine SDS gel and stained for 1 hour.

Unit Definition: One unit is the amount of enzyme that degrades 3 µg of Lambda DNA completely at 37⁰C in 1 minute. Reaction conditions are 50 mM Tris pH 8.0, 6 mM MnSO4.

Storage Buffer: 50 mM Tris pH 8.0, 5 mM MgSO4, 50% Glycerol. Store at -20⁰C.