Traditionally the recombinant lentivirus is purified by ultracentrifugation to separate the virus particles from cellular proteins and media components. The ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed, in addition, the ultracentrifugation also concentrates cellular debris, membrane fragments, and unwanted proteins from culture medium.
The ViraTrapTM lentivirus purification express kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. The viruses are first pelleted from viral supernatant and then further purified and concentrated through a purification filter. The virus can be concentrated 50 -100 folds