Adeno-associated Virus Purification Mini Kit, Serotype 5, 8 and Others, Biomiga

The ViraTrapTM AAV purification kit is designed for fast and efficient purification of AAV  from AAV infected cell culture supernatant, plasma or serum for AAV serotypes 5, 8 and others. 1 x 1012 Viral particles can be purified from cell culture of 1 to 2 T75 flasks per column. The viruses are first applied to a purification column and then further purified and concentrated through a centrifugal filter.

Each column can be regenerated for purifying the same rAAV. For optimized viral binding and recovery, each column can be regenerated only once.

 

ViraTrapTM Adeno-associated Virus Purification Kit Features

Fast gravity flow or spin procedure within 2 hours.

•Purify up to 3x1012 viral particles from 6 to 8 x T75 flask per column.

•No freeze/thaw cycles that may damage virus.

•Uniquely formulated enzyme for removing DNA/RNA.

V1169 (Mini Kit) and V1269 (Maxi Kit) work for serotypes 2 and DJ. 

V1369 works for 5, 8, and others.

 

Kit Components

 

Catalog#

V1369-00

V1369-01

V1369-02

Preps

2

10

20

AAV Columns

1

5

10

Press-On Cap

1

10

20

4 ml Centrifugal Device

4

10

20

Nuclease

10 uL

55 μL

110 μL

10x Nuclease Buffer

100 uL

500 μL

1000 μL

Buffer B

10 mL

35 mL

70 mL

Buffer P

10 mL

70 mL

140 mL

Buffer S

30 mL

100 mL

200 mL