96-well Blood gDNA Purification Kit, Biomiga

 Biomiga 96-Well Blood DNA Kit allows rapid and reliable isolation of high-quality genomic DNA /viral DNA in a high-through-put 96-well format from a wide variety of samples including fresh, frozen, or anticoagulated whole blood, serum, plasma, bone marrow, body fluids, lymphocytes and cultured cells. Blood DNA is bound to Biomiga's ezBind matrix while proteins and other unwanted impurities are removed by two rapid wash. Pure DNA is then eluted from the matrix with Elution Buffer or ddH2O. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. The DNA binding capacity per well is 50 µg.

 

Storage and Stability

All components of the EZgeneTM 96-Well DNA Kit are stable for at least 12 months from date of purchase when stored at 22-25°C. Buffer BL may form precipitates in cool ambient conditions, Warm up the bottle at 37°C to dissolve before use. Store Protease K at -20°C.

 

Kit Contents

Product Number

GD2815-00

GD2815-01

GD2815-02

96-Well DNA Plate

1

4

20

96-Well Collection Plate (2 mL)

1

2

4

Round Well Plate (1.2 mL)

1

4

20

Caps for round-well Plate

24 x 8

96 x 8

480 x 8

Racked Microtubes

1

4

20

Caps for Racked Microtubes

12 x 8

48 x 8

240 x 8

Buffer BL

30 mL

100 mL

500 mL

Protease K

50 mg

200 mg

1.0 g

Buffer KB

45 mL

170 mL

820 mL

DNA Wash Buffer

70 mL

280 mL

5 x 280mL

Elution Buffer

40 mL

160 mL

2 x 250 mL

Sealing Film

5

20

100

Instruction Booklet

1

1

1

 

Storage of Blood Samples

Storage of blood samples without previous treatment leads to reduced yields of genomic DNA. For the best result, blood samples should be treated as follows,

  • l For short-term storage (up to a week), collect blood in tubes containing EDTA as anticoagulant, and store at 4 °C.
  • l For long-term storage, collect blood in tubes contain an anticoagulant and store at -70°C. Thawed frozen blood sample at 37°C with gently agitation before use.

Determination of Yield and Quality

The total DNA yield can be calculated as:

[DNA] = (Absorbance260) x (0.05 μg/μL) x (Dilution factor)

The quality of DNA can be assessed by measuring absorbance at A260/280. A ratio of (A260/A280) of 1.8-1.9 corresponds to 90%-95% purity.