BAC/PAC Maxi Isolation Kit, Biomiga

The EZgeneTM high-capacity-plasmid DNA Isolation Kit is designed for rapid purification of BAC, PAC, cosmid and P1 from bacterial cultures. It is based on a modified alkaline lysis procedure that is specially adapted for spin column. The procedure associated with this kit has been tested using a variety of low copy cosmid, BAC, PAC and P1 in different E. coli strains. In addition, this kit can also be used for high copy plasmid isolation. The expected yield from 200 mL of 2 x YT is around 20 to 80 μg.

 

Storage and Stability

 All EZgeneTM BAC/PAC DNA Isolation Kit components are guaranteed for at least 12 months from the date of purchase when stored as follows: Buffer A1/RNase A mixture at 4°C, and all other materials at 22-25°C.

 

Kit Contents

 

Catalog #

PD1314-00

PD1314-01

PD1314-02

Preps

2

10

25

DNA Columns

2

10

25

Buffer X1

25mL

130 mL

320 mL

Buffer X2

25 mL

130 mL

320 mL

Buffer X3

25 mL

130 mL

320 mL

Buffer KB

12 mL

60 mL

135 mL

Binding Buffer

6 mL

25 mL

70 mL

Elution Buffer

3 mL

15 mL

30 mL

DNA Wash Buffer

6 mL

25 mL

54 mL

RNase A

(20 mg/mL)

3 mg

(150 μL )

13 mg

(700 μL)

32 mg

(1600 μL)

User Manual

1

1

1

* Binding Buffer: Add 24 mL (PD1314-00) or 100 mL (PD1314-01) or 280 mL (PD1314-02) of isopropanol before use. **DNA Wash Buffer: Add 24 mL (PD1314-00) or 100 mL (PD1314-01) or 216 mL (PD1314-02) of absolute ethanol before use.