96 BAC/PAC Isolation Kit, Biomiga

The 96-well BAC or PAC DNA Isolation Kit is designed for rapid high-throughput purification of BACs, PACs, and P1s from small volume of E. Coli.bacterial cultures grown and processed in a 96-well plate format. Process may be performed by using either a centrifugation or full vacuum protocol. One 96-well plate can be processed manually within 60 minutes and two plate in less than 80 minutes. The procedure has been developed and tested using a variety of low copy cosmids, BACs, PACs, P1s , and E. coli strains. This kit can also be used for high copy plasmid isolation.

The 96-well Fastfilter BAC/PAC DNA Isolation procedure is based on modified alkaline lysis procedure in which the bacterial cells are lysed in the presence of RNase A, After the naturalization state, the cell lysates are cleared by filtration using 96-well Lysate Clearance Plate. The liberated BAC, PAC, P1, or plasmid is trapped on to the membrane in the 96-well DNA Plate with a propriety technology. After two quick wash step, the purified BAC, PAC or plasmid DNA can be eluted with low Elution Buffer or water. The eluted BAC, PAC or plasmid can be directly used in downstream applications.

 

Storage and Stability

All components are guaranteed for 24 months from the date of purchase. The Buffer A1/RNase A should be stored at 4oC.

 

Kit Contents

 

Catalog#

PD1312-S

PD1312-01

PD1312-02

Preps

1

4

20

96- Well 2.2 mL

Plate

1

4

20

96- Well 1.6 mL

Plate

1

4

20

96-Well DNA Plate

1

4

20

96-Well Lysate Clearance Plate

1

4

20

96-Well Collection Plate

2

5

24

Ventilating Film

1

4

20

Sealing Film

4

16

80

Buffer X1

30 mL

(Add RNase A before use)

110 mL

(Add RNase A before use)

2 x 300 mL

Buffer X2

30 mL

(Keep tightly capped after use)

110 mL

(Keep tightly capped after use)

2 x 300 mL

Buffer X3

30 mL

(Contain chaotropic salts)

110 mL

(Contain chaotropic salts)

2 x 300 mL

Binding Buffer

8 mL

(Add 32 mL isopropanol before use)

32 mL

(Add 128 mL isopropanol before use)

2 x 80 mL

(Add 320 mL isopropanol before use)

DNA Wash Buffer

50 mL

(Add 200 mL ethanol before use)

2?100 mL

(Add 400 mL ethanol before use)

7 x125 mL

(Add 500 mL ethanol before use)

Buffer KB

55 mL

240 mL

3 x 400 mL

Elution Buffer

25 mL

100 mL

450 mL

RNase A(20mg/mL)

160 ?L

600 ?L

2 x 1.5 mL

Use Manual

1

1

1