Cell/Tissue RNA Purification Mini Kit Plus, Biomiga

Biomiga's EZgeneTM Tissue RNA Kit provides an easy and fast method for isolating total RNA from tissues, cultured cells within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary. This kit purifies up to 100 µg of total RNA from eukaryotic cells or animal tissues. The purified RNA is ready for RT-PCR, Northern blotting, polyA+ RNA purification, nuclease protection, and in vitro translation.

Cell/Tissue RNA Purification Mini Kit Plus provides one set of column to remove genomic DNA and another set of columns for RNA purification

Feateatured Citations  

1.     Nature (2011) doi:10.1038/nature09707

 

 

Features

•  Genomic DNA removed by a gDNA removal column

•  No DNase I digestion needed

•  Procedure takes less 30 minutes

•  Save 40% as compared to competitors

•  Cells, tissue, blood and plant samples
 

Figure 1

Figure 2  Protocol Flow Chart

 

Specifications

 

Protocol Processing Time <30 min.
Starting Sample  5×106 Eukaryotic cells 
  30mg tissue
RNA Yield 100 µg of total RNA
Downstream Applications

RT-PCR

Northern blotting

polyA+ RNA purification

nuclease protection

in vitro translation

Microarray

quantitative gene study

Shipping Condition Room Temperature
Regulatory Statement For Research Use Use Only. Not intended for any animal or human therapeutic or diagnostic use.

 

   

Kit Content

Buffer HLY,Buffer HRB,RNA Wash Buffer,DEPC-Treated ddH2O,ezBind RNA Columns,DNA Clearance Column,Collection Tubes

 

Storage

DNase I (optional) should be stored at -20°C. All other components can be stored at room temperature. All kit components are guaranteed for 12 months from the date of purchasing.

 

 

Regulatory Statement

For Research Use Use Only. Not intended for any animal or human therapeutic or diagnostic use.