endA+ Strains Plasmid Purification Kits, Biomiga

   

Biomiga's Plasmid Purification Kit is a  Breakthrough in Plasmid DNA Purification.

maxi_front_480

 

  • Superior Purity: This patented Plasmid Purification system uses no chaptropic salts in the buffer, and the resulting purified DNA is free of guanidine salts, cesium chloride, ethidium bromide and ion exchange resin residues, which enable the highest performance of downstream applications including gene therapy and genetic vaccination.
  • Ultra High Yield: When comparing Biomiga' Plasmid Purification System to other purification methods, our methodology produces higher DNA yield while requiring fewer bacterial cultures.
  • Time Saving: Using our unique Megaprep Kit, 10 mg plasmid DNA can be purified from 1,000 mL culture within 1 hour!
  • Environment benefit: The EZgene System is the only method available that does not utilize Guanidine Salt, a hazardous protein denaturant, in the silica based purification system, which will ensure the safety and health of scientists and the environment!

 

endA+ Strains Plasmid Purification Kits, Biomiga

These plasmid purification kits are especially designed for purifying plasmid DNA from endA+ strains such as HB101, JM101, TG1 or their derived strains.

Unlike other kits in the markets, Biomiga patented plasmid purification kit has no chaotropic salts in the buffer, the purified DNA is guanidine/anion exchange resin residues free which enable the high performance of downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.

References:

The yield of plasmid DNA depends on the origin of the replication and the size of the plasmid. The protocols are optimized for high copy number plasmid purification. For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 3 to 5 times. Please contact our customer service for further information and reference the table below for the commonly used plasmids,

 

Plasmid

Origin

High copy

Low copy

pACYC

P15A

 

10-12

pSC101

pSC101

 

5

pSuperCos

pMB1

 

10-20

pBR322

pMB1

 

15-20

pUC

Muted pMB1

500-700

 

pGEMR

Muted pMB1

300-400

 

pBluescriptR

ColE1

300-500

 

Citations

Murine Xenograft Model for Human Uterine Fibroids: An In Vivo Imaging Approach.  Reproductive Sciences, 2009, 16(9), 827-842

Characterization of monoclonal antibodies against goat interleukin-18 and their application in the measurement of goat interleukin-18 in LPS-stimulated peripheral blood mononuclear cells by sandwich ELISA. Veterinary Immunology and Immunopathology, Volume 138, Issue 3, 2010, 235-238

Platelet-Derived Growth Factor C Is Upregulated in Human Uterine Fibroids and Regulates Uterine Smooth Muscle Cell Growth1. Biology of Reproduction, 2009, vol. 81 no. 4749-758

Cloning, characterization and tissue distribution of a pi-class glutathione S-transferase from clam (Venerupis philippinarum): Response to benzo[alpha]pyrene exposure. Comparative Biochemistry and Physiology, Part C: Toxicology & Pharmacology , 2010,Volume 152, Issue 2, August 2010, Pages 160-166

An Mpeg (macrophage expressed gene) from the Pacific oyster Crassostrea gigas: Molecular characterization and gene expression. Fish & Shellfish Immunology, 2011, Volume 30, Issue 3, March 2011, 870-876

Construction of eukaryotic recombinant vector of renalase and its expression as a eukaryotic protein. Journal of Shanghai Jiaotong University (Science), 2010, Volume 15, Number 5, 637-640