Plasmid Midi Kit I, Biomiga

Biomiga's Plasmid Purification System

·         Superior Purity:This patented Plasmid Purification system uses no chaptropic salts in the buffer, and the resulting purified DNA is free of guanidine salts, cesium chloride, ethidium bromide and ion exchange resin residues, which enable the highest performance of downstream applications including gene therapy and genetic vaccination.

·         Ultra High Yield:When comparing Biomiga' Plasmid Purification System to other purification methods, our methodology produces higher DNA yield while requiring fewer bacterial cultures.

·         Time Saving:Using our unique Megaprep Kit, 10 mg plasmid DNA can be purified from 1,000 mL culture within 1 hour!

·         Environment benefit:The EZgene System is the only method available that does not utilize Guanidine Salt, a hazardous protein denaturant, in the silica based purification system, which will ensure the safety and health of scientists and the environment!

Citations

Murine Xenograft Model for Human Uterine Fibroids: An In Vivo Imaging Approach.  Reproductive Sciences, 2009, 16(9), 827-842

Characterization of monoclonal antibodies against goat interleukin-18 and their application in the measurement of goat interleukin-18 in LPS-stimulated peripheral blood mononuclear cells by sandwich ELISA. Veterinary Immunology and Immunopathology, Volume 138, Issue 3, 2010, 235-238

Platelet-Derived Growth Factor C Is Upregulated in Human Uterine Fibroids and Regulates Uterine Smooth Muscle Cell Growth1. Biology of Reproduction, 2009, vol. 81 no. 4749-758

Cloning, characterization and tissue distribution of a pi-class glutathione S-transferase from clam (Venerupis philippinarum): Response to benzo[?]pyrene exposure. Comparative Biochemistry and Physiology, Part C: Toxicology & Pharmacology , 2010,Volume 152, Issue 2, August 2010, Pages 160-166

An Mpeg (macrophage expressed gene) from the Pacific oyster Crassostrea gigas: Molecular characterization and gene expression.Fish & Shellfish Immunology, 2011, Volume 30, Issue 3, March 2011, 870-876

Construction of eukaryotic recombinant vector of renalase and its expression as a eukaryotic protein.Journal of Shanghai Jiaotong University (Science), 2010, Volume 15, Number 5, 637-640

 

Biomiga Plasmid Midi Kit

Biomiga Plasmid Midi Kit provides an easy and reliable method to purify plasmid DNA from 50-100 mL bacterial cultures in less than 60 minutes. The DNA is ready for downstream applications such as transfection, RFLP, DNA amplification, and automated sequencing.

  • Biomiga Plasmid Midi Kit I: 100-200 μg of high copy number plasmid DNA can be isolated from 50 mL culture.
  • Biomiga Plasmid Midi Kit II: 200-400 μg of high copy number plasmid DNA can be isolated from 100 mL culture.
  • Choice of 2 types of columns for centrifugation or vacuum manifold.

·        Plasmid ezFilter Midi Kit , Centrifuge

·        Plasmid  ezFilter Midi Kit, Vacuum Manifold

  • Endofree Plasmid Midiprep Kit: Endotoxin < 0.2 EU / μg DNA.

 

Biomiga Plasmid Midi Kit I

Key to Biomiga Plasmid Midi Kit I is Biomiga’s proprietary DNA binding systems that allow DNA exclusively and efficiently bind to our ezBindTM matrix while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer.

Unlike other kits in the markets, our patented plasmid purification kit has no chaotropic salts in the buffers, the purified DNA is guanidine/anion exchange resin residues free which enable the high performance of downstream applications.

This kit is designed for fast and efficient purification of plasmid DNA from 15 to 50 mL of E. coli culture. The midi column has a plasmid DNA binding capacity of 250 µg. The yield from 50 mL culture is typically around 150 to 250 µg. 

 

The purified DNA is ready for high performance of downstream applications such as transfection of robust cells such as HEK293, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.

 

Storage and Stability

Buffer A1 should be stored at 4°C once RNase A is added. All other materials can be stored at room temperature (22-25°C). The Guaranteed shelf life is 12 months from the date of purchase.

 

Before Starting

Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each step.

 Important

  • RNase A: It is stable for more than half a year when stored at room temperature. Spin down the RNase A vial briefly. Add the RNase A solution to Buffer A1 and mix well before use. Store at 4°C.
  • Buffer B1 precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use.
  • Keep the cap tightly closed for Buffer B1 after use.
  • Make sure the availability of centrifuge, especially, after mixing the lysate with ethanol, the sample needs to be processed immediately either by centrifugation.
  • Carry out all centrifugations at room temperature.

 

Materials supplied by users

  • 70% ethanol and 100% ethanol.
  • High speed centrifuge.
  • 30 mL high speed centrifuge tubes.
  • 15 mL and 50 mL conical tubes.
  • 1.5 mL tubes.
  • Isopropanol if precipitate the plasmid DNA.
     

Kit Contents

 

Catalog

PD1411-00

PD1411-01

PD1411-02

Preps

2

10

25

ezBindTM Columns

2

10

25

Buffer A1

6 mL

30 mL

70 mL

Buffer B1

6 mL

30 mL

70 mL

Buffer C1

7 mL

35 mL

85 mL

Buffer KB

7 mL

35 mL

85 mL

RNase A

(20 mg/mL)

0.6 mg(30 µL)

3 mg(150 µL)

7 mg(350 µL)

Elution Buffer

5 mL

25 mL

60 mL

User Manual

1

1

1

 

Safety Information

  • Buffer C1 contains acidic acid, wear gloves and protective eyewear when handling.
  • Buffer C1 and KB contains chaotropic salts, which may form reactive compounds when combines with bleach. Do not add bleach or acidic solutions directly to the preparation waste.

Operating Protocol